10 min readA retrospective study of 42 healthy individuals using Rejuvant (Ca-AKG + gender-specific vitamin) over 7 months showed an average reduction of 8 years in biological age, measured by DNA methylation clocks. The benefit was robust across age and sex and especially pronounced in older participants. This is the first published human evidence showing such a marked age reversal using Rejuvant’s specific form of CaAKG.
This review explores AKG’s role in preventing muscle atrophy and enhancing regeneration. In preclinical and human studies, AKG improves satellite cell proliferation and muscle repair, reduces inflammation through macrophage polarization, and enhances endurance and recovery in active individuals. It also activates mTOR and boosts energy production under stress, highlighting its relevance for aging muscle, injury recovery, and performance support.
AKG directly scavenges reactive oxygen species (ROS) like hydrogen peroxide, converting them into harmless byproducts through oxidative decarboxylation. It also fuels ATP production via the TCA cycle and promotes synthesis of glutamine and glutamate, critical to redox balance. Studies show AKG reduces oxidative stress across organs including brain, liver, and gut—highlighting its therapeutic potential in aging and inflammation-related disorders.
In two large cohorts of middle-aged mice, dietary Ca-AKG extended lifespan (up to 20% in females) and compressed morbidity—delaying frailty more than it extended life. Mice showed lower inflammation, increased IL-10 production (a key anti-inflammatory cytokine), improved coat condition, and better gait. These findings support AKG’s role in modulating age-related inflammatory signaling (SASP) without eliminating senescent cells.
This review outlines how AKG regulates multiple hallmarks of aging: it modulates mTOR and ATP synthase, supports stem cell function, and modulates DNA/histone demethylation. In C. elegans, flies, and mice, AKG supplementation increases lifespan and healthspan. In humans, AKG levels decline ~10x from age 40 to 80, suggesting that supplementation could restore critical metabolic and epigenetic functions lost with age.
AKG supports gut integrity by improving energy supply to enterocytes and preserving mucosal structure during stress, trauma, or malnutrition. Compared to glutamine, AKG is more stable, easier to formulate, and provides similar benefits: reducing intestinal permeability, lowering ammonia toxicity, and supporting immune activity in the gut. Clinical trials show potential for AKG in post-surgical nutrition, critical illness recovery, and inflammatory bowel disease.
This review summarizes AKG’s multifunctional roles in humans: antioxidant, nitrogen buffer, mitochondrial fuel, and epigenetic regulator. Evidence in animals shows AKG extends lifespan, reduces systemic inflammation, preserves muscle mass, and improves bone density. The authors highlight AKG’s broad potential across muscle loss, cognitive decline, cardiovascular risk, and immune aging.
AKG levels are tightly linked to kidney health. This paper reviews evidence that AKG levels drop in chronic kidney disease, diabetic nephropathy, and ischemic injury. In models, AKG improves acid-base balance, supports antioxidant defenses, and modulates autophagy. It also helps maintain stem cell pools and suppresses fibrosis by influencing collagen synthesis. The authors propose AKG as both a potential biomarker and therapeutic target in renal aging and injury.
In a randomized, placebo-controlled trial, untrained young men supplementing with AKG showed significantly better training tolerance, greater power output, and improved muscle torque after four weeks of exercise. AKG also improved stress-recovery markers. The authors attribute this to AKG’s role in buffering ammonia and supporting energy metabolism, without the catabolic side effects associated with high-dose amino acid intake.
AKG plays a critical role in early stem cell fate decisions. In primed human pluripotent stem cells, added AKG accelerates differentiation into multiple lineages by promoting DNA and histone demethylation. This effect is independent of ATP production, highlighting AKG’s role as an epigenetic cofactor. These findings suggest that AKG could support regeneration and repair by influencing stem cell behavior and improving tissue homeostasis in aging.
This study demonstrated that dietary CaAKG supplementation significantly delayed reproductive aging in female mice. CaAKG extended the fertility window, preserved follicle count, and improved oocyte quality in older mice. Mechanistically, it suppressed the mTOR pathway and oxidative stress, both known to accelerate ovarian aging. The findings suggest that CaAKG supports reproductive longevity, with potential implications for aging-related decline in human fertility.
In cultured human dermal fibroblasts, AKG increased type I procollagen production by over 25%. It activated prolidase, a key enzyme that recycles proline for collagen synthesis, and served as a cofactor for prolyl-4-hydroxylase, critical for forming stable collagen triple helices. In a mouse model, topical AKG application significantly reduced UVB-induced wrinkle formation over 12 weeks, performing comparably to retinoic acid. These findings support AKG as a promising collagen-boosting and anti-aging compound.
Join the thousands of individuals who are serious about healthspan extension. Get insights
into our story and the science that sets us apart.
We use cookies to enhance your experience, analyze site usage, and provide personalized content. You can choose to accept all cookies or reject non-essential ones. For more details, see our Privacy Policy.